Cellular & Molecular Biology Letters

International Scientific Journal
Established 1996

Volume 6 (2001) No. 2A

Volume 6 (2001) pp 251
Title	PREFACE
Authors	Prof. dr hab. Stanislaw Przestalski
	The idea of the Conference "Biophysics and Biology of Environmentally Important Membrane-Active Compounds" was to emphasize the importance of investigations on the interaction of various pollutants on biological membranes. It was an exceptional conference, to the best of my knowledge the first of that kind to be held in the world. And thus it was in a sense experimental. Judging by the interest it inspired and especially by the group of excellent lecturers who came to give presentations, the experiment was a success. The importance of studies on membrane-active compounds, as reported in this publication (based on lectures and posters), comes from the fact that the contact of all substances with living organisms begins at the cell membrane. The contact can occur in two different ways: Harmful substances can affect the membrane directly, disturbing its structure. All changes in membrane structure find their reflection in cell functions (which results from the dependence between the physical structure and biological properties of the membrane). Disturbance in membrane structure, or its integrity, results in the disorganization of the cell function and may eventually lead to cell death. Toxic substances may permeate the cell membrane without affecting it and exert their harmful action inside the cell. In both cases, there exists a contact with the membrane. Thus knowledge of the mechanisms of compound - membrane contacts can be crucial for preventing that poisonous action. The literature on the structure and function of biological membranes is immense. There is perhaps even more literature on the effects of xenobiotics on living organisms traeated as a whole, or on their macroscopic fragments. It is strange how little is known about the interaction of many toxic substances with membranes. Though that issue is well recognized in phamacology (with respect to drugs) and neurology (although much remains to be done in this field), very little is known about the effects of most pollutants on membranes. Since protection of the environment is a global issue, studies on the interaction mentioned are becoming more and more important problem, both for pure scientific and practical reasons. I hope that both the Conference and Conference Materials will contribute to the development of research in the discussed area and result in fruitful collaborations

Volume 6 (2001) pp 255-263
Title	DYNAMICAL AND STRUCTURAL PROPERTIES OF LIPID MEMBRANES IN RELATION TO LIPOSOMAL DRUG DELIVERY SYSTEMS
Authors	Kent Jø Rgensen^1,2, Pernille Hø Yrup¹, Tina B. Pedersen² and Ole G. Mouritsen¹
Abstract	The structural and dynamical properties of DPPC liposomes containing lipopolymers (PEG-lipids) and charged DPPS lipids have been studied in relation to the lipid membrane interaction of enzymes and peptides. The results suggest that both the lipid membrane structure and dynamics and in particular the appearance of small-scale lipid structures might be of importance for the activity of membrane associated and liposome degrading enzymes as well as for the membrane interaction of acylated peptides. The combined experimental and simulation results are of relevance for a rational development of peptide loaded liposomal drug delivery systems that become destabilized by membrane degrading phospholipase A2 enzymes, which are found at elevated concentrations at diseased sites.
Adress and Contact Informations	¹Department of Chemistry, Building 207, Technical University of Denmark, DK-2800, Lyngby; ²Department of Pharmaceutics, The Royal Danish School of Pharmacy, Universitetsparken 2, DK-2100, KÅbenhavn ø , Denmark

Volume 6 (2001) pp 265-269
Title	THE RESPONSE OF THE NEURONAL MEMBRANE TO ACETALDEHYDE TREATMENT
Authors	Anton Liopo¹, Olga Chumakova¹, Ilya Zavodnik¹, Aksana Andreyeva¹, Maria Bryszewska² and Sergey Chizhik³
Abstract	To assess the effects of acetaldehyde (AA) on the native synaptosomal membranes, we used the atomic force microscopy (AFM) and fluorescent methods. A wide range of concentrations (from 2 to 4000 mM) of AA was studied. The visualization of synaptosomes by AFM showed structural changes in the synaptosomal surface at an AA concentration of 50 mM, which is comparable with the AA concentrations occurring during ethanol intoxication. In our study, we observed that AA at small concentrations (up to 50 mM) considerably decreased the microviscosity of the hydrophobic region of the bulk membrane lipids (the ratio of the monomeric and excimeric probe fluorescence decreased). These findings correlate with the AFM study on the effect of 50 mM AA on the washed synaptosome membranes.
Adress and Contact Informations	¹Institute of Biochemistry, Academy of Sciences of Belarus, BLK 50, 230017 Grodno Belarus, ²Department of General Biophysics, University of ó odz, 90-237 ó odz, Poland, ³Metal-Polymer Research Institute, Academy of Sciences of Belarus, 32A Kirov St., Gomel 246050, Belarus

Volume 6 (2001) pp 271-275
Title	THE HEMOLYTIC TOXICITY OF SOME NEW AMINOPHOSPHONATES
Authors	Halina Kleszczynska, Janusz Sarapuk And Dorota Bonarska
Abstract	A series of ten aminophosphonate derivatives were assayed for their hemolytic activity as a preliminary screening for the detection of herbicides. The data obtained indicate: 1. A clear correlation between the hemolytic capacity of the test compounds and their plant growth inhibition and an increase in membrane fluidity was demonstrated. 2. It was found that the most active compounds revealed at least one of the following structural features: an iso-propyl substituent at the phosphorus atom, a tert-butyl group attached to their hexane ring or a long hydrocarbon chain. 3. Ring substituents at the phosphorus (phenyl ring), carbon or nitrogen atoms (hexane) removed the hemolytic activity of compounds. 4. It may be concluded that the hemolytic toxicity of the aminophosphonates studied is related to their ability to incorporate and fuse into the lipid phase of the erythrocyte membrane. The general conclusion is that both stereochemistry and hydrophobicity are deciding factors for the efficiency of the interaction of the studied compounds studied with erythrocytes, and that the most possible location of the aminophosphonates is in the lipid phase of the RBC membrane.
Adress and Contact Informations	Department of Physics and Biophysics, Agricultural University, Wroc”aw, Norwida 25, 50-375, Poland

Volume 6 (2001) pp 277-281
Title	METAL ION-FLAVONOID ASSOCIATIONS IN BILAYER PHOSPHOLIPID MEMBRANES
Authors	Monika Soczynska-Kordala¹, Anna Bakowska1, Jan Oszmianski¹ and Janina Gabrielska²
Abstract	The complexation process of the transition (Cu²⁺, Fe²⁺) and heavy (Pb²⁺) metals with flavonoids (quercetin and rutin) was studied. The investigation was conducted using the spectrophotometric method in a medium containing phosphatidylcholine liposome membrane and in methanol. The Benesi-Hildebrand method was used to determine the constants of flavonoid- metal complex formation of the ”charge transfer„ type, and the Gibbs free energy change for that process. It was found that both quercetin and rutin are very effective at complexing transition atoms in both methanol and in the lipid bilayer. Pb(II) ions were only complexed in methanol. A short discussion is given on the importance of metal coordination processes in the context of lipid membrane peroxidation.
Adress and Contact Informations	¹Department of Friut and Vegetable Technology, ²Department of Physics and Biophysics, Agricultural University, Norwida 25, 50-235 Wroclaw, Poland

Volume 6 (2001) pp 283-290
Title	X-RAY DIFFRACTION AND NEUTRON SCATTERING STUDIES OF AMPHIPHILE - LIPID BILAYER ORGANIZATION
Authors	Pavol Balgavy¹, Daniela Uhrikova¹, Janka Karlovska¹, Martina Dubnic Kova¹, Norbert Kuc Erka¹, Ferdinand Devinsky¹, Ivan Lacko¹, Jozef C Izmarik¹, Karl Lohner², Gabor Degovics², Gert Rapp³, Sergej Yaradaikin⁴, Michail Kiselev⁴, Achmed Islamov⁴ And Valentin Gordeliy⁴
Abstract	The lipid bilayer thickness d_L, the transbilayer distance of lipid phosphate groups d_PP and the lipid surface area AL of fluid hydrated bilayers of lamellar phases of egg phosphatidylcholine or dipalmitoylphosphatidylcholine containing N-alkyl-N,N-dimethylamine N-oxides (CnNO), 1,4-butanedi- ammonium-N,N≥-dialkyl-N,N,N≥,N≥-tetramethyl dibromides (GSn) or mono- hydrochlorides of [2-(alkyloxy)phenyl]-2-(1-piperidinyl)ethylesters of carbamic acid (CnA) were obtained by X-ray diffraction, and the bilayer thickness in extruded unilamellar dioleoylphosphatidylcholine vesicles containing C12NO was obtained by the neutron scattering. The values of d_L, d_PP and A_L change linearly up to the 1:1 amphiphile:lipid molar ratio. The slopes of these dependencies increase for dL and dPP and decrease for A_L with an increasing number of carbons n in the amphiphile long hydrocarbon substituent (18” n” 8 for CnNO, 16” n” 9 for GSn, 12” n” 5 for CnA), while the opposite trends are observed for the short substituent (8” n” 6 for CnNO, 9” n” 7 for GSn, 5” n” 3 for CnA). In case of long substituents, the effects on d_L, d_pp and A_L are caused by the decrease in the difference between the lipid and amphiphile hydrocarbon chain lengths and by the increase in their van der Waals attraction. The short substituent amphiphiles are mobile and exchange between multiple binding sites in the bilayer, minimizing the bilayer surface area.
Adress and Contact Informations	¹Faculty of Pharmacy, Comenius University, SK-832 32 Bratislava, Slovakia, ²Institute of Biophysics and X-Ray Structure Research, Austrian Academy of Sciences, A-8042 Graz, Austria, ³Max Planck Institute for Colloid and Interface Science Golm/Potsdam, c/o HASYLAB, DESY, D-22603 Hamburg, Germany, ⁴Frank's Laboratory of Neutron Physics, Joint Institute for Nuclear Research, 141980 Dubna, Russia

Volume 6 (2001) pp 291-297
Title	THE MEMBRANE-DISRUPTING ACTIVITY OF a- AMINOALKANEPHOSPHONIC ACIDS AND THEIR DERIVATIVES
Authors	Agnieszka Deron¹, Anna Dziamska², Izabela Pawlaczyk¹, Krzysztof Bielecki³ , Halina Kleszczyn Ska², Roman Gancarz¹ and Janusz Sarapuk²
Abstract	The influence of a series of acyclic and cyclic aminophosphonates on the physicochemical properties of model (planar lipid membranes Ń BLM) and biological (erythrocytes Ń RBC) membranes was studied. The results obtained were compared with the results of physiological tests performed on the aquatic plant Spirodela oligorrhiza. It was found that the inhibition of plant growth by the compounds studied correlated, although not very highly, with the observed changes in the properties of membranes used. It was also found that both the biological activity of aminophosphonates and their efficiency at modifying the physicochemical parameters of membranes depended on their structural features.
Adress and Contact Informations	¹Department of Organic Chemistry, Biochemistry and Biotechnology, Wroclaw University of Technology, Wybrzeze Wyspian skiego 27, 50-370 Wrocżaw, Poland, ²Department of Physics and Biophysics, Agricultural University, Wrocżaw, Norwida 25, 50-375, Poland, ³Department of Botany and Plant Physiology, Agricultural University, Wrocżaw, Cybulskiego 32, 50-205 Wrocżaw, Poland

Volume 6 (2001) pp 299-304
Title	THE ACTION OF MERCURY ON CELL MEMBRANES
Authors	Milan Schara¹, Marjana Nemec¹, Ingrid Falnoga¹, Alfred Bogomir Kobal², Marina Kveder³ and Jelka Svetek¹
Abstract	The action of mercuric chloride and methyl mercuric chloride on the membrane lateral domain organization of bovine, equine, and canine erythrocytes was studied. Electron paramagnetic resonance (EPR) spectra of spin-labeled erythrocytes were analyzed with respect to their lateral domain structure. Continuous alteration of the membrane domain populations revealed that mercuric compounds affect the membrane via the evolution of toxic events in the cells.
Adress and Contact Informations	¹J. Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia, ²Mercury Mine Idrija, Arkova ul. 43, 5280 Idrija, Slovenia, ³Rudjer Boskovic Institute, Bijeni“ ka 54, 10000 Zagreb, Croatia

Volume 6 (2001) pp 305-311
Title	MEMBRANE ACTIVE COMPOUNDS THAT AFFECT THE SHAPE OF CELLS AND CELLULAR ORGANELLES
Authors	Sas A Svetina and Bos Tjan Zeks
Abstract	Amphiphilic membrane active compounds are considered that affect the shapes of cells and cellular organelles by intercalation into the phospholipid part of their membranes. It is taken into consideration that amphiphile- membrane interaction modifies membrane mechanical properties. The relationship between membrane mechanical properties and vesicle shapes and the concept of the bilayer couple model are shortly reviewed. Then it is put forward that the strength of the amphiphile-membrane interaction may depend on the lateral packing of phospholipid molecules. It is shown that in such a case the amphiphile molecules bind to the membrane in a cooperative manner. Moreover, the amphiphile binding makes the ratio between the nonlocal and local membrane bending constants to be effectively larger and thus widens the range of possible stable vesicle and cellular shapes.
Adress and Contact Informations	Institute of Biophysics, Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia and J. Stefan Institute, 1000 Ljubljana, Slovenia

Volume 6 (2001) pp 313-318
Title	CYTOKININ ACTIVITIES IN CELLS OF WHEAT INFLORESCENCE IN DEPEPENDENCE OF ITS DEVELOPMENTAL STAGE
Authors	Izabela Marcinska¹*, Maria Filek¹, Jolanta Biesaga- Koscielniak¹, Ferenc Sagi² and Tibor Bartok²
Abstract	We demonstrate that differences in the endogenous biologically active levels and patterns of cytokinins depend on the organ differentiation, i. e. immature inflorescence before meiosis and immature embryo 14 days after anthesis, respectively, in winter wheat of two genotypes: Grana and Almari. Two cell lines were subjected to analysis of cytokinin contents. Higher levels of endogenous cytokinin were found in cells derived from immature embryo (E) in comparison to cells obtained from immature inflorescence (I). The levels of zeatin ryboside ([9R]Z) and benzyladenosine ([9R]BAP) were predominant and isopentenyladenine (iP) was very low for the cells E of both genotypes. The cytokinin amounts in cells E and I of the zeatin group were smaller than those of other groups. We suggest that the concentration of cytokinins in tissue capable of generative development in winter wheat should be on a level that occurred in inflorescence cell lines, in comparison to immature embryo cell lines. These results indicate that the effect of the developmental stage of ears of wheat on the capability to flower in winter wheat plants regenerated in in vitro culture may be induced by the hormone level.
Adress and Contact Informations	¹Department of Plant Physiology, Polish Academy of Sciences, Podżuz na 3, Krakow 30-239, Poland, ²Cereal Research Non-Profit Company, H-6701 Szeged P.O. Box 391, Hungary

Volume 6 (2001) pp 319-326
Title	INTERACTION OF CHANNEL-BLOCKING BISPYRIDINIUM COMPOUNDS WITH SUPPORTED PHOSPHOLIPID LAYERS
Authors	Andrew Nelson¹, Norman Geddes² and John Tattersall³
Abstract	The interaction of phospholipid monolayers of dioleoyl phosphatidylcholine (DOPC) on mercury electrodes with bispyridinium compounds is reported in this paper. The interaction is related to the modification of the capacitance-potential plot and in particular of two well defined phase transitions of the phospholipid monolayer. The order of the extent of interaction of the test compounds with the monolayer can be related to their structure and is:- P65>Toxogonin>BPE>HS6>TMB4>HI6>BPT>P2S. The penetration of the compound into the monolayer depends on potential. At potentials more negative than the occurrence of the two phase transitions, the test compounds penetrate further and disrupt the monolayers. At more positive potentials this effect is reversed.
Adress and Contact Informations	¹School of Chemistry, University of Leeds, Leeds LS2 9JT, UK, ²DERA, Malvern, UK, 3DERA, Porton Down, Wiltshire, UK

Volume 6 (2001) pp 328-428
Abstracts of the lectures
Abstracts List	PORE-FORMING TOXINS: ATTACK AND DEFENCE AT THE CELL SURFACE C. Lindsay Bashford - p.328 BIOMEMBRANE PERTURBATION INDUCED BY XENOBIOTICS IN MODEL AND LIVING SYSTEMS Enrico Bertoli, Annarina Ambrosini, Giovanna Zolese, Rosita Gabbianelli, Donatella Fedeli and Giancarlo Falcioni - p.334 INTERACTION OF ORGANOTIN COMPOUNDS WITH PHOSPHOLIPID MEMBRANES José J. Chicano, Antonio Ortiz, José A. Teruel and Francisco J. Aranda - p.340 ENVIRONMENTAL TOXINS AND ION CHANNELS Krzysztof Dołowy - p.343 BIOPHYSICAL APPLICATION OF THE OPTICAL WAVEGUIDE LIGHTMODE SPECTROSCOPY Róbert Horváth, György Fricsovszky and Elemér Papp - p.348 NATURAL AMPHIPHILIC PHENOLS AS BIOACTIVE COMPOUNDS Arkadiusz Kozubek, Robert Żarnowski, Maria Stasiuk and Jerzy Gubernator - p.351 THE H+-PUMPING V-ATPASE OF HIGHER PLANTS: A VERSATILE "ECO-ENZYME"; IN RESPONSE TO ENVIRONMENTAL STRESS Ulrich Lüttge, Elke Fischer-Schliebs and Rafael Ratajczak - p.356 COMPOUNDS THAT MODULATE MULTIDRUG RESISTANCE IN CANCER CELLS Krystyna Michalak, Andrzej Hendrich, Olga Wesołowska and Andrzej Poła - p.362 UNASSISTED AND ASSISTED ION TRANSPORT ACROSS MEMBRANES: INSIGHTS FROM COMPUTER SIMULATIONS Andrew Pohorille and Michael A. Wilson - p.369 SELENIUM COMPOUNDS IN THE ENVIRONMENT; THEIR EFFECT ON HUMAN HEALTH Barbara Wachowicz, Halina M. Żbikowska and Paweł Nowak - p.375
Poster Abstracts
Abstracts List	THE EFFECT OF ALUMINIUM ON THE STABILITY OF INTRACELLULAR MEMBRANES Anna Banasik and Anna Lankoff - p.384 THE PHYSIOLOGICAL ACTIVITY OF NEW AMINOPHOSPHONATES Krzysztof Bielecki, Zenon Trela, Anna Dziamska and Janusz Sarapuk - p.385 THE EQUILIBRIUM OF PHOSPHATIDYLCHOLINE-CHOLESTEROL IN MONOLAYERS AT THE AIR/WATER INTERFACE Izabela Brzozowska and Zbigniew A. Figaszewski - p.386 ARE LUNAR RHYTHMS A "ZEITGEBER" FOR CIRCUMNUTATIONS IN SUNFLOWER PLANTS (HELIANTHUS ANNUUS)? Agnieszka Buda, Maciej Krupa, Tadeusz Zawadzki, Kazimierz Trębacz, Halina Dziubińska and Elżbieta Król - p.387 SODIUM AND HYDROGEN IONS AS MODULATORS OF SYNAPTOSOMAL MEMBRANE TRANSPORT ACTIVITY Olga Chumakova and Ilya Zavodnik - p.389 EFFECTS OF WASTE INCINERATOR FLY ASH ON PROINFLAMMATORY RESPONSES OF PULMONARY MACROPHAGES AND EPITHELIAL CELLS Silvia Diabaté, Sonja Mülhopt, Hanns-Rudolf Paur and Harald F. Krug - p.390 THE INFLUENCE OF GREEN TEA ON ETHANOL INTOXICATION- INDUCED CHANGES IN RAT LIVER CELL MEMBRANES Izabela Dobrzyńska, Barbara Szachowicz-Petelska and Zbigniew A. Figaszewski - p.391 AN ESTIMATION OF THE POTENTIAL ACTIVITY OF NEWLY-SYNTHESIZED ACYCLIC ORGANOPHOSPHOROUS COMPOUNDS Anna Dziamska, Agnieszka Deron, Roman Gancarz and Janusz Sarapu - p.392 THE INTERACTION OF ZEARALENOE AND STEARIC ACID IN ARTIFICIAL CELLULAR MEMBRANES Maria Filek, Izabela Marcińska and Jolanta Biesaga-Kościelniak - p.393 A COMPARISON OF THE TRI- AND TETRAETHYLS OF LEAD AND TIN AS MEMBRANE MODIFIERS AND CHLORIDE CARRIERS ACROSS LIPID BILAYERS Andrzej Fogt and Stanisław Przestalski - p.394 SPECTROPHOTOMETRIC ANALYSIS OF ORGANISATION OF POLYENE ANTIBIOTIC AMPHOTERICIN B IN LIPID MEMBRANES Mariusz Gagoś, Roman Koper and Wiesław I. Gruszecki - p.395 THE STABILIZATION OF FMN DIMERS BY POLYVINYL ALCOHOL FILM Hanna Grajek and Grażyna Żurkowska - p.396 THE ROLE OF PHENOTHIAZINE RING SUBSTITUTION STRUCTURE IN THE INTERACTION OF PHENOTHIAZINE DERIVATIVES WITH ZWITTERIONIC LIPIDS Andrzej B. Hendrich, Olga Wesołowska, Noboru Motohashi and Krystyna Michalak - p.397 THE STABILITY AND REACTIVITY OF SOME TRIALKYL-TIN AND TRIALKYL-LEAD HYDROXYLATES. AN INVESTIGATION USING MOLECULAR QUANTUM MECHANICS METHODS Jerzy Hładyszowski - p.398 EFFECT OF EXOGENOUS NO ON RESPONSES OF THE NON-PREGNANT HUMAN MYOMETRIUM TO ENDOTHELIN-1 Tomasz Kleszczewski, Beata Modzelewska and Anna Kostrzewska - p.399 THE INTERACTION OF SOME LYSOSOMOTROPIC SUBSTANCES WITH MEMBRANE ERYTHROCYTE Halina Kleszczyńska, Dorota Bonarska, Jacek Łuczyński and Stanisław Witek - p.400 THE MODULATION OF MULTIDRUG RESISTANCE BY NEWLY-SYNTHESIZED PHENOTHIAZINE DERIVATIVES Marcin Kołaczkowski, Krystyna Michalak and Noboru Motohashi - p.401 THE EFFECT OF NITRIC OXIDE ON HUMAN INTRAUTERINE ARTERY RESPONSES TO VASOPRESSIN Anna Kostrzewska - p.402 STATIONARITY ANALYSIS OF CHRONOPOTENTIOMETRIC TIME SERIES FROM BILAYER LIPID MEMBRANE SUBJECTED TO ELECTROPORATION Małgorzata Kotulska, Stanisława Koronkiewicz and Sławomir Kalinowski - p.403 THE ANTIFUNGAL ACTIVITY OF DMAL AND PYG CLASS LYSOSOMO-TROPIC DRUGS RESULTED FROM THEIR EFFECT ON CELL MEMBRANES Anna Krasowska, Lucyna Chmielewska, Jacek Skała, Karel Sigler, Jacek Łuczyński and Stanisław Witek - p.404 LOW-TEMPERATURE INDUCED TRANSMEMBRANE CHANGES OCCURING ON THE PLASMALEMMA AND THE TONOPLAST OF THE LIVERWORT Conocephalum conicum Elżbieta Król, Halina Dziubińska, Kazimierz Trębacz, Tadeusz Zawadzki, Maciej Krupa and Agnieszka Buda - p.405 ALKYLATED METAL COMPOUNDS MIMIC SIGNAL MOLECULES: THE INDUCTION OF PROGRAMMED CELL DEATH VIA MEMBRANE RECEPTORS Harald F. Krug, Peter-Christian Klöhn, Martin Göttlicher and Peter Herrlich - p.406 SHOULD MODEL RESEARCH ON LIPID MEMBRANES TAKE INTO ACCOUNT THE DIPOLE CHARACTER OF LIPID POLAR HEADS? Krystian Kubica - p.408 THE EFFECT OF CADMIUM ON GROWTH, PROTON SECRETION AND MEMBRANE POTENTIAL IN MAIZE COLEOPTILE CELLS Renata Kurtyka and Waldemar Karcz - p.409 THE EFFECT OF CYANOBACTERIAL TOXINS ON THE STABILITY OF INTRACELLULAR MEMBRANES Anna Lankoff and Anna Banasik - p.410 THE MODIFICATION OF PHOTOELECTRIC PHENOMENA BY SOME IONS OF HEAVY METALS IN PLANT CELLS Barbara Michalczuk, Jan Stolarek and Krystyna Pazurkiewicz-Kocot - p.411 THE INFLUENCE OF EXOGENOUS NITRIC OXIDE ON THE POTASSIUM CHANNELS OF THE HUMAN NON-PREGNANT MYOMETRIUM Beata Modzelewska, Tomasz Kleszczewski and Anna Kostrzewska - p.413 THE CAPACITANCE AND RESISTANCE OF THE TWO-COMPONENT BILAYER LIPID MEMBRANE MODELLING OF THE CELL MEMBRANE Monika Naumowicz, Aneta D. Petelska and Zbigniew A. Figaszewski - p.414 QUATERNARY AMMONIUM SALTS AND THEIR CORRESPONDING AMINES AS GROWTH INHIBITORS OF BAKER�S YEAST Saccharomyces cerevisiae Ewa Obłąk, Tadeusz M. Lachowicz, Sylwia Bartosik, Jacek Łuczyński and Stanisław Witek - p.415 THE AGGREGATION OF LUTEIN IN LIPID MEMBRANES � THE MONTE CARLO SIMULATION Witold Okulski - p.416 THE INFLUENCE OF PLANT GLYCOSAMINOGLYCANS ON ERYTHROCYTE MEMBRANES Izabela Pawlaczyk, Dorota Bonarska, Halina Kleszczyńska and Roman Gancarz - p.417 THE EFFECT OF pH ON THE INTERFACIAL TENSION OF BILAYER LIPID MEMBRANES FORMED FROM PHOSPHATIDYLCHOLINE OR PHOSPHATIDYLSERINE Aneta D. Petelska, Monika Naumowicz and Zbigniew A. Figaszewski - p.419 THE EFFECT METALLIC IONS AND pH ON THE DYNAMIC PROPERTIES OF LIPID MEMBRANES Marian Podolak, Dariusz Man and Monika Jedynak - p.420 SECOND DERIVATIVE SPECTROPHOTOMETRIC DETERMINATION OF THE PARTITION COEFFICIENTS OF NEWLY-SYNTHESIZED PHENOTHIAZINES BETWEEN THE PHOSPHATIDYLCHOLINE PHASE AND WATER Andrzej Poła, Anna Burliga, Krystyna Michalak and Noboru Motohashi - p.421 THE INTERACTION OF PHENYLTIN COMPOUNDS WITH MODEL MEMBRANES IN THE PRESENCE OF SURFACTANTS Bożenna Różycka-Roszak and Hanna Pruchnik - p.423 CHANGES IN THE PHYSICO-CHEMICAL PROPERTIES OF HUMAN LARGE INTESTINE TUMOUR CELL MEMBRANES Barbara Szachowicz-Petelska, Izabela Dobrzyńska,Zbigniew A. Figaszewski and Stanisław Sulkowski - p.424 EXTRACELLULAR ZINC INHIBITS THE ACTIVITY OF Kv1.3 CHANNELS IN HUMAN T LYMPHOCYTES Andrzej Teisseyre and Jerzy W. Mozrzymas - p.425 THE EFFECT OF PHOSPHATE ESTER DERIVATIVES OF POLYPRENOL ON MODEL MEMBRANE STABILITY Krystyna Walińska and Tadeusz Janas - p.426 PHENOTHIAZINE MALEATES � PUTATIVE MDR MODIFIERS � DECREASE THE FLUIDITY OF MODEL PHOSPHATIDYLSERINE MEMBRANES Olga Wesołowska, Andrzej B. Hendrich, Noboru Motohashi and Krystyna Michalak - p.427 THE EFFECT OF POLYPHENOLIC COMPOUNDS ON THE FUNCTIONAL PROPERTIES OF RAT LIVER MICROSOMES TREATED WITH LOW-DOSE gamma-IRRADIATION Leu B. Zavodnik, Alina P. Shkodich, Vladimir A. Ovchinnikov, Svetlana N. Ilina, Aleksey A. Deniskovec and Vyacheslav U. Buko - p.428
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