Volume 10 (2005) pp 499-514 |
Abstract |
We constructed and analyzed a new cell line called HT5-1, which
stably expresses an enhanced green fluorescent protein-tagged version of the rat
vanilloid receptor 1 (VR1/TRPV1). The fluorescent receptor allowed easy
measurement of receptor expression and expression level-based purification of
cells via fluorescence-activated cell sorting. The HT5-1 cell line was compared
to cells transiently transfected with the fluorescent receptor, to cells expressing
the native rat vanilloid receptor, and to isolated capsaicin-sensitive rat trigeminal
sensory neurons. Fura-2 microfluorimetry measurements of the calcium influx
upon capsaicin induction showed that, by contrast to transiently transfected cells,
HT5-1 cells respond uniformly to the stimulation, due to the similar level of
receptor expression in individual cells. HT5-1 cells showed similar behaviour to
isolated trigeminal root ganglion neurons, including marked tachyphylaxis upon
repeated capsaicin induction, and a lack of calcium ion release from intracellular
storage sites. |
Address and Contact Information |
1Department of Pharmacology and Pharmacotherapy, Faculty of Medicine,
Neuropharmacology Research Group of the Hungarian Academy of Sciences,
University of Pécs,, Szigeti u. 12, H-7624 Pécs,, Hungary, 2Department of
Pharmacology and Pharmacotherapy, Medical and Health Science Center,
University of Debrecen, Nagyerdei krt. 98, H-4032, Debrecen, Hungary,
3Department of Biophysics, Faculty of Medicine, University of Pécs,,
Szigeti u. 12, H-7624 Pécs,, Hungary * Corresponding author; tel: 36-72-536217, fax: 36-72-536218, e-mail:
janos.szolcsanyi@aok.pte.hu |