Volume 9 (2004) pp 805 - 817 |
Abstract |
A set of Thatcher near-isogenic lines and two breeding lines were
used to examine sequence tagged site (STS) markers linked to leaf rust
resistance genes Lr9, Lr10, Lr19, Lr24, Lr28, Lr29, Lr35, and a simple
sequenced repeat (SSR) marker for Lr39. The selected STS markers for
resistance genes Lr9, Lr10, Lr19, Lr24 and Lr28 were identified in seven
accessions by seven European laboratories. Near-isogenic lines of the spring
wheat Thatcher were used as positive controls. Markers for resistance genes Lr9,
Lr10, Lr19, Lr24 were identified in all seven laboratories as amplification
products of 1100 bp, 310 bp, 130 bp and 310 bp, respectively. The STS markers
linked to resistance genes Lr9, Lr10, Lr19, Lr24, Lr29, Lr35 and the SSR marker
for Lr39 were robust and highly specific for these genes and will be useful in
marker-assisted selection in wheat. However, the amplification product of 378
bp that corresponded with resistance gene Lr28 was detected in all accessions including genotypes lacking this gene in all seven laboratories. This marker
needs to be improved. |
Address and Contact Information |
1Institute of Plant Genetics Polish Academy of Sciences, Strzeszyłska 34,
60-479 Poznań Poland, 2Lochow-Petkus GmbH, PF 1197, D-29296 Bergen
Germany, 3Division of Genetics and Plant Breeding, Research Institute of Plant
Production, Bratislavská cesta 122, 92168 Pieštany, Slovakia, 4Institute of
Epidemiology and Resistance, Federal Center for Breeding Research on Cultivated
Plants, Theodor-Roemer-Weg 4, 06449 Aschersleben, Germany,
5Research Institute of Crop Production, Department of Molecular Biology, Drnovská 507,161 06 Praha 6 - Ruzyně, Czech Republic, 6Cereal Research
Nonprofit Company, 6726 Szeged Also-Kikoto sor 9, Hungary, 7Hungarian
Academy of Sciences Plant Protection Institute, Budapest, Hungary * Corresponding author; e-mail: jche@igr.poznan.pl |