Vol. 5 No. 2 June 2000

Volume 5 (2000) pp 145-150
Title THE UNIQUE CYCLOPROPANE FATTY ACID COMPOSITION OF RHIZOSPHERAL Pseudomonas fluorescent ISOLATES
Authors Robert Zarnowski1,*, Stanislaw J. Pietr1, José F. Quilez2, Eduardo Cabrera2 and Alejandro F. Barrero2
Abstract 9,10-methylenehexadecanoic acid was found to be the main and sole constituent of the cyclopropane fatty acid (CFA) pool of five Pseudomonas fluorescent strains. Our findings have shown that all the studied isolates of plant-growth promoting rhizospheral fluorescent pseudomonads have qualitatively identical CFA profiles, even though they have the ability to make use of distinct carbon sources and show different plant root colonisation ability. This report suggests that the preservation of the proper CFA composition in biomembranes may be crucial for rhizobacteria adaptation to the fluctuation of physicochemical conditions in the rhizosphere
Address and Contact Information 1Agricultural University of Wrocław, Department of Agricultural Microbiology, ul. Grunwaldzka 53, 50-375 Wrocław, Poland,
2University of Granada, Faculty of Science, Department of Organic Chemistry, 18071 Granada, Spain
* - To whom offprint requests should be sent: Robert Żarnowski, Katedra Mikrobiologii Rolniczej, Akademia Rolnicza, ul. Grunwaldzka 53, 50-375 Wrocław, Polska. E-mail: robert@ozi.ar.wroc.pl
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Volume 5 (2000) pp 151-169
Title THE PROPERTIES OF PLANT (Cucurbita pepo) NUCLEAR MATRIX PREPARATIONS ARE STRONGLY AFFECTED BY THE PREPARATION METHOD
Authors Ryszard Rzepecki
Abstract Nuclear matrices from White bush (Cucurbita pepo var. patissonina) cell nuclei were isolated. Three different preparation methods were used. The methods were: I- the method of Berezney and Coffey [1] involving extraction of cell nuclei with 2M NaCl and Triton X-100 (called the “High Salt” method); II- the same with pre-treatment of cell nuclei with 0.5 mM CuSO4 (stabilisation step); III- the method of Mirkovitch et al. [2] involving lithium diiodosalicylate (LIS) extraction (called the “LIS” method). Each of the three methods was used in three variants of nucleic acid removal: restriction enzymes, endogenous nucleases and DN-ase I with RN-ase A digestion. Nuclear matrices were analysed for protein and DNA content, residual RNA and DNA synthesis activity, endonucleolytic activity and specific SAR DNA binding properties. The lowest protein and DNA content and endonucleolytic activity was found in nuclear matrices isolated by the “High Salt” method. It also had the lowest RNA and DNA synthesis and endonucleolytic activity. The highest protein and DNA content, and RNA and DNA synthesis and endonucleolytic activity was found in nuclear matrices isolated by the “LIS” method. When exogenous SAR DNA binding activity was compared, the highest was found in nuclear matrices isolated by the “High Salt” method while the lowest was in the “LIS” method preparation. Nuclear matrices isolated by the “High Salt” method with a stabilisation step always displayed average values of assayed parameters. These data indicate that the biological residual properties of a nuclear matrix preparation strongly depend on the method used.
Address and Contact Information Department of Genetic Biochemistry, Institute of Biochemistry and Molecular Biology, University of Wrocław, Przybyszewskiego Str. 63/77, 51-148 Wrocław, Poland
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Volume 5 (2000) pp 171-190
Title THE ANTIMUTAGENIC ACTIVITY OF TWO PLANT-DERIVED COMPOUNDS.A COMPARATIVE CYTOGENETIC STUDY.
Authors Kazimierz Gąsiorowski 1,#, Barbara Brokos1, Arkadiusz Kozubek2 and Jan Oszmiałski3
Abstract The antimutagenic activity of alkylresorcinols from cereal grains and anthocyanins from Aronia melanocarpa fruit were compared in three short-term lymphocyte tests: a sister chromatid exchange test, a cytokinesis-blocked micronucleus assay and a thioguanine-resistance test. It was noticed that both tested compounds significantly decreased the rate and frequency of mutations induced in cultured lymphocytes with two standard mutagens: benzo[a]pyrene and mitomycin C. Alkylresorcinols appeared to be more potent antimutagenic compounds than anthocyanins. Anthocyanins exhibited a stronger inhibitory effect on the generation and release of free radicals by human granulocytes in vitro, as measured with the NBT-reduction test. The results suggest that alkylresorcinols and anthocyanins exerted an antimutagenic influence through multifarious mechanisms, one of which could be a limitation of free radical involvement in mutagenesis.
Address and Contact Information 1 Wrocław Medical University, Department of Basic Medical Sciences, 14 Kochanowskiego Str., 51-601 Wrocław, Poland,
2 University of Wrocław, Department of Lipids an Liposomes, 63/77 Przybyszewskiego Str., 51-148 Wrocław, Poland,
3 Agricultural University, Department of Fruit and Vegetable Technology, 25 Norwida Str., 51-375 Wrocław, Poland
#Corresponding author, fax : (+48 71) 3479211, e-mail: kaz@basmed.am.wroc.pl
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Volume 5 (2000) pp 191-206
Title RIBOSOME BIOGENESIS AND NUCLEOLAR FUNCTION IN YEAST Saccharomyces cerevisiae
Authors Robert Gromadka and Joanna Rytka*
Abstract This review provides an overview of the current knowledge of ribosome biogenesis, nucleolus structure and function and protein traffic into and out of the nucleus, with emphasis on the potential of yeast Saccharomyces cerevisiae as a model organism.
Address and Contact Information Institute Biochemistry and Biophysics, Polish Academy of Sciences 5A Pawinskiego Str., 02-106 Warsaw, Poland
*Corresponding author
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Volume 5 (2000) pp 207-230
Title 37kDa ONCOFETAL ANTIGEN IS AN AUTOIMMUNOGENIC HOMOLOGUE OF THE 37kDa LAMININ RECEPTOR PRECURSOR
Authors Adel L. Barsoum, James W. Rohrer and Joseph H. Coggin, Jr
Abstract The laminin receptor precursor (LRP) has been the center of intense controversy and interest for over a decade. Chief among these controversies is the putative multifunctional aspect of this molecule: as a ribosomal-associated protein (p40) and/or as the precursor for the 67kDa high-affinity laminin receptor (67LR), neither of which have been definitively established. Othercontroversiesincludethe presence of more than one 67kDa laminin receptor protein. However, interest in the molecule is sustained because of its vital function in the cell as a component of the translational machinery and due to its constant up-regulation in cancer cells with the invasive and metastatic phenotype and in association with poor prognosis. Recently, while studying the autoimmunogenicity of a species conserved 37-44 kDa oncofetal antigen (37kDa OFA), the authors of this paper, using amino acid sequencing of isolated OFA-peptides, cDNA cloning and sequencing and antigens- antibodies cross reactivity as well as antigens-T cell recognition reactions, identified the 37kDa OFA as precursor or immature laminin receptor protein.
Address and Contact Information University of South Alabama College of Medicine, Department of Microbiology and Immunology, Mobile, AL 36688
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Volume 5 (2000) pp 232-284
Seminar Title W. MEJBAUM-KATZENELLENBOGEN'S MOLECULAR BIOLOGY SEMINARS: 7. CHROMATIN ARRANGEMENT AND PROGRAMMED CELL DEATH
Abstracts List THE ROLE OF 14-3-3 PROTEINS IN NEURODEGENERATIVE DISEASES, TRAFFICKING AND NUCLEAR SIGNALLING
Alastair Aitken, Helen C. Baxter, Jane Forster and Thierry Dubois - [Rozmiar: 1332 bajtĂłw]p.232-233

LAW AND ORDER IN THE NUCLEUS: DYNAMICS OF REPLICATION FACTORIES IN LIVING CELLS
Maria Cristina Cardoso, Hans-Peter Rahn, Anje Sporbert and Heinrich Leonhardt - [Rozmiar: 1332 bajtĂłw]p.234

DNA DAMAGE AND APOPTOSIS INDUCTION IN L1210 CELLS BY CIS-DIAMMINEDICHLOROPLATINUM(II) AND ITS NEW AMINOFLAVONE ANALOGUE
Ewa Ciesielska, Kazimierz Studzian, Elżbieta Zyner, Justyn Ochocki and Leszek Szmigiero - [Rozmiar: 1332 bajtĂłw]p.235

APOPTOTIC POLYKARYONS, CHROMATIN DIMINUTION AND SURVIVAL MECHANISM IN POLYPLOID GIANT CELLS DERIVED FROM P53 MUTANT LYMPHOMA CELL LINES AFTER GENOTOXIC DAMAGE
Jekaterina Erenpreisa, Mark Steven Cragg , Roger Alston , Anton Page and Timothy Martin Illidge - [Rozmiar: 1332 bajtĂłw]p.236-237

NUCLEAR ENVELOPE LIMITED CHROMATIN SHEETS IN P53 MUTATED BURKITT'S LYMPHOMA CELL LINES AFTER DNA AND SPINDLE DAMAGE
Jekaterina Erenpreisa, Andrey Ivanov, Mark Steven Cragg , Galina Selivanova and Timothy Martin Illidge - [Rozmiar: 1332 bajtĂłw]p.238

ORGANISATION OF THE CHROMATIN FIBRE IN THE YEAST ARTIFICIAL CHROMOSOMES CONTAINING HUMAN DNA
Anna Fiszer-Kierzkowska, Jacek Krol, Grzegorz Ira and Jan Filipski - [Rozmiar: 1332 bajtĂłw]p.239-240

DNA TOPOISOMERASE II, INTRAGENIC RECOMBINATION AND CHROMOSOMAL GENE ORGANIZATION
Mickail V. Glazkov and Julia A. Mechtcheriakova - [Rozmiar: 1332 bajtĂłw]p.241

NUCLEAR LAMINS: MAJOR DETERMINANTS OF NUCLEAR ARCHITECTURE
Robert D. Goldman, Anne Goldman, Reynold Lopez-Soler, Robert D. Moir and Timothy Spann - [Rozmiar: 1332 bajtĂłw]p.242

CASPASE-MEDIATED CLEAVAGE OF THE CHROMATIN-BINDING DOMAIN OF LAMINA- ASSOCIATED POLYPEPTIDE LAP2?
Josef Gotzmann, Sylvia Vlcek and Roland Foisner - [Rozmiar: 1332 bajtĂłw]p.243

THE NUCLEAR LAMINA: NEW INSIGHTS FROM WORMS AND FLIES
Yosef Gruenbaum - [Rozmiar: 1332 bajtĂłw]p.244

DEDICATED SITES OF GENE EXPRESSION IN THE NUCLEI OF MAMMALIAN CELLS
Dean A. Jackson, Francisco Iborra, Ana Pombo and Hiroshi Kimura - [Rozmiar: 1332 bajtĂłw]p.245

INVESTIGATIONS OF RADIATION INDUCED APOPTOSIS IN HCV-29 CELLS AND THEIR V-RAS AND V-RAF TRANSFECTANTS
Dariusz Kowalczyk, Joanna Miłoszewska and Przemysław Janik - [Rozmiar: 1332 bajtĂłw]p.246

PROXIMAL PART OF HUMAN TYROSINE HYDROXYLASE GENE BINDS NUCLEAR MATRIX IN TISSUE SPECIFIC MANNER
Robert Lenartowski and Anna Goc - [Rozmiar: 1332 bajtĂłw]p.247

M/SAR PROPERTIES OF BOVINE DNA ENCLOSING THE TYROSINE HYDROXYLASE GENE
Robert Lenartowski, Tomasz Grzybowski, Danuta Miścicka-Śliwka and Anna Goc - [Rozmiar: 1332 bajtĂłw]p.248

THE POLY(ADP-RIBOSYL)ATION SYSTEM: TISSUE SPECIFICITY AND AGE-RELATED CHANGES IN RATS
Maria Malanga, Carmelita D'Ambra, Anna Petrella, Angela Ferone, Roy Jones and Benedetta Farina - [Rozmiar: 1332 bajtĂłw]p.249-250

STUDY OF CELL SURVIVAL IN NON ADHERENT CONDITIONS
Maciej Małecki, Paulina Sroczyńska and Przemysław Janik - [Rozmiar: 1332 bajtĂłw]p.251

APOPTOTIC EFFECT OF CYANOBACTERIAL HEPATOTOXIC EXTRACTS FROM POLISH RESERVOIRS
Joanna Mankiewicz, Zofia Walter, Małgorzata Tarczyłska, Maciej Zalewski, Kari Espolin Fladmark and Stein Ove Doskeland - [Rozmiar: 1332 bajtĂłw]p.252

MOLECULAR CHARACTERISATION OF RETINOBLASTOMA PROTEIN INTERACTIONS WITH THE NUCLEAR LAMINS
Ewa Markiewicz, Rachel Venables, Roy Quinlan and Christopher J. Hutchison - [Rozmiar: 1332 bajtĂłw]p.253-255

EXPRESSION OF TGF-beta RECEPTORS AND SMAD PROTEINS IN HUMAN ENDOMETRIAL CANCERS
Dagmara Piestrzeniewicz, Andrzej Semczuk, Magdalena Bryś, Hanna Romanowicz-Makowska, Jerzy A. Jakowicki and Wanda M. Krajewska - [Rozmiar: 1332 bajtĂłw]p.256

DETECTION AND CHARACTERIZATION OF RAT PROTEINS RECOGNIZING AND BINDING DNA DAMAGED BY N-ACETOXY-ACETYLOAMINOFLUORENE AND CIS-PLATINUM
Monika Pietrowska, Joanna Łanuszewska, Joanna Rzeszowska-Wolny and Piotr Widłak - [Rozmiar: 1332 bajtĂłw]p.257

B-CELL CHRONIC LYMPHOCYTIC LEUKEMIA-ASSOCIATED NUCLEAR ANTIGENS
Małgorzata Rogalińska, Jerzy Z. Błoński, Agata Szuławska, Joanna Chruściel, Hanna Niewiadomska, Tadeusz Robak and Zofia M. Kiliańska - [Rozmiar: 1332 bajtĂłw]p.258

BIOCHEMICAL MECHANISMS OF APOPTOTIC EXECUTION
Sandrine Ruchaud, Kumiko Samejima, Françoise Durrieu and William Earnshaw - [Rozmiar: 1332 bajtów]p.259

THE ISOLATED 14-3-3 PROTEIN ISOFORMS PROTECT DNA AGAINST ENDONUCLEASE(s) DIGESTION IN VITRO
Ryszard Rzepecki, Ewa Szałowska, Anna Klimek, Grzegorz Wilczyński and Jan Szopa - [Rozmiar: 1332 bajtĂłw]p.260

INTERACTIONS OF Drosophila melanogaster LAMIN Dm WITH NUCLEIC ACIDS AND TOPOISOMERASE II IN VIVO
Ryszard Rzepecki and Paul A. Fisher - [Rozmiar: 1332 bajtĂłw]p.261-262

THE STUDY OF Drosophila melanogaster LAMIN Dm PHOSPHORYLATION IN TISSUE CULTURE Kc CELLS IN VIVO
Ryszard Rzepecki and Paul A. Fisher - [Rozmiar: 1332 bajtĂłw]p.263

ORGANISATION AND COMPOSITION OF THE PLANT NUCLEAR MATRIX. CHARACTERISATION AND SUBCELLULAR DISTRIBUTION OF A MAR-BINDING PROTEIN: AcMFP1
Rafael Samaniego and Susana Moreno Diaz de la Espina - [Rozmiar: 1332 bajtĂłw]p.264-266

NON-LYMPHOID CULTURED CELLS POSSESS A SYSTEM CONTROLLING CELLULAR COMPATIBILITY
Lidia P. Sashchenko, Elena A. Dukhanin, Elena S. Ioudinkova, Olga V. Iarovaia, Tamara I. Lukianova and Sergey V. Razin - [Rozmiar: 1332 bajtĂłw]p.267-268

RB PROTEIN IS EXPRESSED IN HUMAN ENDOMETRIAL CARCINOMAS
Andrzej Semczuk, Regine Schneider-Stock, Roman Miturski, Danuta Skomra, Jacek Tomaszewski, Marek Gogacz, Albert Roessner and Jerzy Jakowicki - [Rozmiar: 1332 bajtĂłw]p.269

CYTOGENETIC ANALYSIS OF THE CHROMOSOME REGIONS WHICH ATTACH TO THE NUCLEAR ENVELOPE
Igor V. Sharakhov - [Rozmiar: 1332 bajtĂłw]p.270

A NOVEL APOPTOSIS-LIKE CELL DEATH, INDEPENDENT OF CASPASE-3, INDUCED BY CURCUMIN
Ewa Sikora - [Rozmiar: 1332 bajtĂłw]p.271-272

PROBING NUCLEAR ENVELOPE, PORE, LAMINA ASSEMBLY AND CHECKPOINT MECHANISMS IN METAZOANS
Carl Smythe, Hazel Jenkins, Sheona Drummond, Clare Hall-Jackson, Barry Marshall, Carmen Feijoo and Christopher J. Hutchison - [Rozmiar: 1332 bajtĂłw]p.273-274

METHYL-CPG-BINDING PROTEIN 2 (MECP2): ROLE IN DEVELOPMENT AND GENE EXPRESSION
Wolf H. Strätling, Fang Yu, Jens Thiesen and Jan Buschdorf - [Rozmiar: 1332 bajtĂłw]p.275

PROLIFERATING CELL NUCLEAR ANTIGEN IN COUMESTROL TREATED HUMAN ENDOMETRIAL STROMAL CELLS CULTURE
Jacek Tomaszewski, Andrzej Semczuk, Roman Miturski and Jerzy Jakowicki - [Rozmiar: 1332 bajtĂłw]p.276

LAMINS A AND C FORM A STRUCTURAL COMPLEX THAT ANCHORS EMERIN AT THE NUCLEAR ENVELOPE
Owen A.Vaughan, Mauricio Alvarez-Reyes, Joanna M. Bridger, Jos L.V. Broers, Manfre Wenhert, Glen E. Morris, William G.F.Whitfield and Christopher J. Hutchison - [Rozmiar: 1332 bajtĂłw]p.277

MOLECULAR MARKERS OF APOPTOSIS AS PROGNOSTIC INDICATORS IN CANCER
Zbigniew Walaszek and Malgorzata Hanausek - [Rozmiar: 1332 bajtĂłw]p.278-279

ROLE OF POLY(ADP-RIBOSE) POLYMERASE IN THE REGULATION OF THE STABILITY OF WILD-TYPE p53 PROTEIN AND p53-MEDIATED APOPTOSIS
Jozefa Wesierska-Gadek - [Rozmiar: 1332 bajtĂłw]p.280-281

THE ROLE OF DFF40/CAD ENDONUCLEASE DURING TERMINAL STAGES OF APOPTOSIS
Piotr Widłak - [Rozmiar: 1332 bajtĂłw]p.282

STUDYING OF 14-3-3 PROTEIN FUNCTION IN TRANSGENIC POTATO
Grzegorz Wilczyński, Anna Kulma and Jan Szopa - [Rozmiar: 1332 bajtĂłw]p.283

ANALYSIS OF RABBITS FED ON WILD TYPE AND GENETIC MODIFIED POTATO TUBERS
Magdalena Żuk, Anna Kulma, Grzegorz Wilczyłski, Anna Prescha and Jan Szopa - p.284 [Rozmiar: 1332 bajtĂłw]

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