Volume 9 (2004) pp 577-587 |
Title |
RESVERATROL PROTECTS AGAINST PEROXYNITRITE-INDUCED THIOL OXIDATION IN BLOOD PLATELETS |
Authors |
Beata Olas, Paweł Nowak and Barbara Wachowicz |
Abstract |
The peroxynitrite anion (ONOO·) is a reactive species produced in the
reaction between the superoxide anion (O2··) and nitric oxide (·NO). ONOO· is
involved in several pathological conditions such as inflammation,
arteriosclerosis, and neurodegenerative and cardiovascular disorders. Our earlier
results showed that ONOO· inhibits different steps of blood platelet activation
and causes the depletion of platelet thiols. In this study, we investigated the
effects of resveratrol (3, 4', 5-trihydroxystilbene) and other antioxidants (uric
acid and deferoxamine (DFO)) on the level of low molecular thiols such as
glutathione, cysteine and cysteinylglycine (in reduced and oxidized form) in
blood platelets treated with ONOO·. Our results showed that ONOO· (100 mM,
2 min) induces changes in these thiols (measured by HPLC method); these
changes are diminished in the presence of resveratrol. Preincubation of human
platelets with resveratrol at a concentration of 100 mM (30 min) has a protective
effect against the oxidation of platelet thiols induced by ONOO· or its
intermediate. The other tested antioxidants also have a protectory action. In
conclusion, we suggest that the resveratrol present in the human diet may
partially protect -SH groups from oxidation and may be responsible for redox
regulation and control in platelets. |
Address and Contact Information |
Department of General Biochemistry, University of Łódź, Banacha 12/16,
90-237 Łódź, Poland |
|
Volume 9 (2004) pp 589-602 |
Title |
VESICLES WITH A DOUBLE BILAYER |
Authors |
Zygmunt H. Zawada |
Abstract |
A modified reverse phase evaporation method was used to prepare
intermediate unilamellar vesicles coated with an additional membrane, or large
vesicles in which several vesicles were coated with a common membrane. In
both kinds of vesicle, the outer and inner membranes are usually of different
phospholipid composition. The preparation involves the formation of a double
emulsion: vesicles in a buffer are emerged in a low-boiling point organic
solution of phospholipids. Then the organic solvent is evaporated during the
heating and mixing process. As result large unilamellar vesicles (LUVs), about
100 nm in diameter, were coated with an additional membrane from egg lecithin
or dipalmitoylphosphatidylcholine and cholesterol. The highest yield of the
coating was about 50%. When DPPC was used for coating above the phase
transition temperature Tm, the data suggested the formation of vesicles that were
slightly larger than the starting LUVs. It might be concluded that many of these
had a double bilayer. If the coating was done below Tm , the micrographs
suggested the formation of structures resembling multi-vesicular vesicles. They
looked like LUV clusters coated with a common membrane. |
Address and Contact Information |
Department of Physical Pharmacy, Medical University of Silesia,
ul. Jagiellołska 4, 41-200 Sosnowiec, Poland |
|
Volume 9 (2004) pp 603-615 |
Title |
A SINGLE-STEP METHOD OF LIPOSOME PREPARATION |
Authors |
Zygmunt H. Zawada |
Abstract |
All the liposome preparation protocols, which involve drug
encapsulation are multi-step processes, i.e. they consist of one or several steps of
preparation and homogenization. The conditions of converting all lipids into
vesicles smaller than 200 nm were determined by replacing ultrasonication with
mechanical stirring of the buffer and solution of lipids in a low-boiling point
organic solvent or solvents in a simple preparator. Preferably, the process should
be carried out at a temperature higher than the temperature of the gel/fluid phase
transition (Tm), and higher than the boiling point of the organic solvent(s) used
to obtain the lipid solution. For many lipid membrane compositions, the products
of preparation are as follows: a dominant fraction of unilamellar vesicles (vesicle
of diameter smaller than 200 nm) and a fraction of much larger multivesicular or
multilamellar vesicles, easily separated by simple centrifugation at 15000´g.
If PEG-phosphatidylethanolamine or cholesteryl palmitate are additional
membrane components, multivescular or multilamellar vescicles are virtually
absent in the final product, of a single-step process and all the used lipids were
quantitatively converted into vesicles smaller than 200 nm in diameter. |
Address and Contact Information |
Department of Physical Pharmacy, Medical University of Silesia, Jagiellołska 4,
41-200 Sosnowiec, Poland |
|
Volume 9 (2004) pp 617-634 |
Title |
A SIMPLE METHOD FOR THE ESTIMATION OF RECOMBINATION FREQUENCIES AND GENETIC DISTANCES |
Authors |
Cesar Benito and Araceli Gallego |
Abstract |
We have developed an alternative approach to the maximum
likelihood method for calculating recombination values and linkage intensities.
This new method is simpler than those in current use in that it obviates the need
for formulae and tables. Maximum likelihood methods imply the use of iterative
procedures over highly complicated estimation equations (at least second degree
polynomials), whereas simplified methods use single-step procedures involving
simple linear equations. The proposed method uses the frequencies of the
distinguishable phenotypes that came from the union of at least one recombinant
gamete with another gamete. We performed Monte-Carlo simulations for
various combinations of genetic distance and offspring size. The recombination
values obtained using the new method were compared with those derived by the
maximum likelihood method on both simulated and experimental data. They
were found to be nearly identical. The observed distribution of the
recombination frequency values does not differ significantly from the Normal
distribution, except for extreme values of the mean, as the Skewedness and
kurtosis coefficients do not differ from zero. Our method has a similar accuracy
to the maximum likelihood methods for recombination frequencies smaller than
25 cM and the difference does not increase greatly for greater frequencies. |
Address and Contact Information |
Department of Genetics, Faculty of Biology, University Complutense,
28040-Madrid, Spain
|
|
Volume 9 (2004) pp 635-641 |
Title |
THE MARKERS OF OXIDATIVE STRESS AND ACTIVITY OF THE ANTIOXIDANT SYSTEM IN THE BLOOD OF ELDERLY
PATIENTS WITH ESSENTIAL ARTERIAL HYPERTENSION |
Authors |
Kornelia Kędziora-Kornatowska1, Jolanta Czuczejko2, Hanna Pawluk2, Tomasz Kornatowski3, Jadwiga Moty1, Leszek Szadujkis-Szadurski3, Karolina Szewczyk-Golec3 and Józef Kędziora2,4 |
Abstract |
We estimated the nitrate/nitrite, carbonyl groups, reduced glutathione
(GSH) and malondialdehyde (MDA) concentrations and Cu,Zn superoxide
dismutase (SOD-1), catalase (CAT), glutathione peroxidase (cGSH-Px) and
glutathione S-transferase (GST) activities in the blood of 17 normotensive young
subjects (mean age 39±7.0 years), 21 normotensive elderly subjects (mean age
82±8.2 years) and 38 patients with essential arterial hypertension (mean age
73±8.0 years). Our examinations showed that hypertension in the elderly is
associated with greater than normal levels of protein and lipid oxidation,
decreased nitric oxide concentration and an imbalance in antioxidant status
(decreased GSH concentration and SOD-1 activity). The increased activity of
GST compensated the decreased activity of cGSH-Px in the blood of
hypertensive patients. Our study confirms that the degree of oxidative stress in
elderly patients intensifies, especially if said patients have associated essential
arterial hypertension. |
Address and Contact Information |
1Department and Clinic of Geriatrics, L. Rydygier Medical Academy of
Bydgoszcz, Curie Skłodowskiej 9, 85-094 Bydgoszcz, Poland, 2Department of
Biochemistry, L. Rydygier Medical Academy of Bydgoszcz, Poland,
3Department of Pharmacology and Therapy, L. Rydygier Medical Academy of
Bydgoszcz, Poland, 4Department of Clinical Biochemistry, Medical University
of Łódź, Poland
*Corresponding author; tel: +48 (52) 5854021, fax: +48 (52) 5854921,
e-mail: kasiakor@interia.pl |
|
Volume 9 (2004) pp 643-650 |
Title |
OXIDATIVE STRESS INDUCES IGF-I RECEPTOR SIGNALING DISTURBANCES IN CULTURED HUMAN DERMAL FIBROBLASTS.
A POSSIBLE MECHANISM FOR COLLAGEN BIOSYNTHESIS INHIBITION |
Authors |
Paweł Sienkiewicz, Maciej Pałka and Jerzy Pałka* |
Abstract |
The effects of oxidative stress on collagen and DNA biosynthesis, b-
galactosidase and prolidase activities, and the expression of prolidase, b1-
integrin receptor, FAK, IGF-IR and MAP-kinases (ERK1, ERK2) were evaluated
in human dermal fibroblasts. Subconfluent cells were subjected to repetitive
stresses with 30mM t-BHP for 1 hour per day over the course of 5 days. It was
found that oxidative stress induced the inhibition of collagen biosynthesis in
these cells in a time-dependent manner. Exposure of the cells to 5 stresses
contributed to a decrease in collagen and DNA biosynthesis to about 30% and
50% of the control values, respectively. Prolidase activity and expression were
only suppressed in fibroblasts subjected to 1 and 3 stresses. In these cells
prolidase activity was decreased by about 20%. As a result of 5 stresses, no
further inhibition of prolidase activity occurred; however, expression of the
enzyme was slightly increased, as demonstrated by Western blot analysis. It was
found that these phenomena were neither related to the expression of b1-integrin
receptor nor to that of FAK. However, the exposure of the cells to 3 and 5
stresses contributed to a distinct decrease in IGF-IR and MAP-kinases (ERK1,
ERK2) expression, which is probably responsible for the collagen biosynthesis
inhibition. |
Address and Contact Information |
Department of Medicinal Chemistry Medical Academy of Białystok,
ul. Kilinskiego 1, 15-230 Białystok, Poland
*Corresponding author; tel: +48 (85) 7485706. fax: +48 (85) 7485416,
e-mail: pal@amb.edu.pl. |
|
Volume 9 (2004) pp 651-664 |
Title |
A CHARACTERIZATION OF THE ACTIVITIES OF IRON REGULATORY PROTEIN 1 IN VARIOUS FARM ANIMAL SPECIES |
Authors |
Rafał R. Starzyński1, Mikołaj A. Gralak2, Ewa Smuda 1 and Paweł Lipiński1 |
Abstract |
Iron regulatory protein 1 (IRP1) post-transcriptionally regulates the
expression of proteins involved in the iron metabolism of mammals. IRP1 is a
bifunctional cytosolic protein which can exhibit aconitase activity or bind to iron
responsive element (IREs) in the untranslated regions of specific mRNAs. The
modulation of IRP1 activities and its consequence for intracellular iron
homeostasis is best characterized in rodents and humans. Little is known about
IRP1 in farm animals. In this study, we analyzed the two activities of IRP1 in the
livers of four farm animal species (cattle, goat, pig and rabbit) and their
relationship to hepatic iron content. We found an inverse correlation between
spontaneous IRP1 IRE binding activity and non-haem iron content in the liver.
Using the electrophoretic mobility shift assay, we showed differential mobility
of IRE/IRP1 complexes formed with hepatic cytosolic extracts from various
farm animal species. We discuss this observation in relation to a comparative
analysis of mammalian IRP1 amino acid sequences. |
Address and Contact Information |
1Institute of Genetics and Animal Breeding, Polish Academy of Sciences,
Jastrzębiec, Poland, 2Department of Physiological Sciences, Faculty of
Veterinary Medicine, Warsaw Agricultural University, Warszawa, Poland |
|
Volume 9 (2004) pp 665-673 |
Title |
TEMPORAL PATTERNS OF LIPID PEROXIDATION PRODUCT FORMATION AND ANTIOXIDANTS ACTIVITY
IN ORAL CANCER PATIENTS |
Authors |
Arul Albert Baskar, Shanmugam Manoharan, Thamilarasan Manivasagam and Perumal Subramanian* |
Abstract |
The aim of this study was to assess the temporal patterns of (the
formation of) thiobarbituric acid reactive substances and the activities of
antioxidant enzymes in the erythrocytes of ten healthy adult subjects and ten oral
cancer patients of comparable age. The levels of thiobarbituric acid reactive
substances and the activities of antioxidant enzymes were assayed at 6-hour
intervals using colorimetric methods. The Cosinorwin computer software
program was used to analyse the characteristics of biochemical rhythms, such as
acrophase, amplitude, and mesor (rhythms: acrophase, amplitude, mesor, etc.).
There is a phase delay in erythrocyte thiobarbituric acid reactive substance levels
and enzymatic antioxidant activities in oral cancer patients as compared to
healthy subjects. The desynchronisation of thiobarbituric acid reactive substance
production and enzymatic antioxidant rhythms reflected an alteration of
circadian clock function in oral cancer patients and may require specific
measures for chronotherapy. |
Address and Contact Information |
Department of Biochemistry, Faculty of Science, Annamalai University,
Annamalai Nagar - 608 002, Tamil Nadu, India
* Corresponding author; tel: 0091-04144-238343-extn: 212, fax: 0091-04144-238080,
e-mail: psub@rediffmail.com |
|
Volume 9 (2004) pp 675-683 |
Title |
THE SENSITIVITY OF YEAST AND YEAST-LIKE CELLS TO NEW LYSOSOMOTROPIC AGENTS |
Authors |
Anna Krasowska1, Lucyna Chmielewska1, Ryszard Adamski1, Jacek Łuczyłski2, Stanisław Witek2 and Karel Sigler3 |
Abstract |
The lysosomotropic action of the compounds DM-11 and DMAL-12s
against Saccharomyces cerevisiae, Schizosaccharomyces pombe and Candida
albicans is species- and pH-dependent. At pH 6.0, DMAL-12s is less effective
against S. cerevisiae and S. pombe but more effective against C. albicans than
DM-11. At pH 8.0, DMAL-12s strongly inhibits the growth of S. cerevisiae but
has only a marginal effect on the resistant C. albicans. S. pombe did not grow at
pH 8.0. As shown by quinacrine accumulation, DM-11 causes a general
intracellular acidification in all three species, while with DMAL-12s, the
acidification is marginal. Morphological changes caused by DMAL-12s in
S. cerevisiae affect the cell interior but not surface structures, while S. pombe
cells exhibit a thickened and wrinkled cell wall, shrunken protoplast and
“grainy” plasma membrane. A large number of blisters resembling lipid droplets
were observed inside S. cerevisiae and S. pombe vacuoles. The high
susceptibility of S. pombe cells to the action of DM-11 and DMAL-12s contrasts
with the low sensitivity of S. pombe H+-ATPase to the agents. In our C. albicans
isolate, DMAL 12s did not have an effect on cell morphology and appeared to be
unable to penetrate the cells, especially at pH 8.0. |
Address and Contact Information |
1Institute of Genetics and Microbiology, Wrocław University, Przybyszewskiego
63-77, 51-148 Wrocław, Poland, 2Dept. of Chemistry, Technical University of
Wrocław, Wyb. Wyspiałskiego 27, 50-370 Wrocław, Poland, 3Institute of
Microbiology, Acad. Sci. Czech Republic, Vídeňská 1083, 142 20 Prague 4,
Czech Republic
* Corresponding author: e-mail: aniak@microb.uni.wroc.pl
|
|
Volume 9 (2004) pp 685-697 |
Title |
GENETIC DIVERSITY ANALYSIS IN VALENCIA PEANUT (Arachis hypogaea L.) USING MICROSATELLITE MARKERS |
Authors |
Girish Kumar Krishna1, Jinfa Zhang2, Mark Burow3, Roy N. Pittman4, Stanko G. Delikostadinov4, Yingzhi Lu1 and Naveen Puppala1,5 |
Abstract |
Cultivated peanut or groundnut (Arachis hypogaea L) is an important
source of oil and protein. Considerable variation has been recorded for
morphological, physiological and agronomic traits, whereas few molecular
variations have been recorded for this crop. The identification and understanding
of molecular genetic diversity in cultivated peanut types will help in effective
genetic conservation along with efficient breeding programs in this crop. The
New Mexico breeding program has embarked upon a program of improvement
of Valencia peanut (belonging to the sub species fastigiata), because efforts to
improve the yield potential are lacking due to lack of identified divergent exotic
types. For the first time, this study has shown molecular diversity using
microsatellite markers in the cultivated Valencia peanut (sub spp. fastigiata)
from around the globe. In this investigation, 48 cultivated Valencia peanut
genotypes have been selected and analyzed using 18 fluorescently labeled SSR
(f-SSR) primer pairs. These primer pairs amplified 120 polymorphic loci among
the genotypes screened and amplified from 3 to 19 alleles with an average of 6.9
allele per primer pair. The f-SSR marker data was further analyzed using cluster
algorithms and principal component analysis. The results indicated that (1)
considerable genetic variations were discovered among the analyzed genotypes;
(2) The f-SSR based clustering could identify the putative pedigree types of the
present Valencia types of diverse origins, and (3) The f-SSR in general is
sufficient to obtain estimates of genetic divergence for the material in study. The
results are being utilized in our breeding program for parental selection and
linkage map construction. |
Address and Contact Information |
1New Mexico State University, Department of Agronomy and Horticulture, Las
Cruces, NM 88003, U.S.A, 2Texas A&M Research Center, Lubbock, TX,
3USDA-ARS, Griffin, GA, 4Institute for Plant Genetic Resources, Sadovo -
Bulgaria,
5Agricultural Science Center at Clovis, NM 88101, U.S.A.
* Corresponding author: e-mail: npuppala@nmsu.edu
|
|
Volume 9 (2004) pp 699-707 |
Title |
ENHANCED LIPID PEROXIDATION AND IMPAIRED ENZYMIC ANTIOXIDANT ACTIVITIES IN THE ERYTHROCYTES
OF PATIENTS WITH CERVICAL CARCINOMA |
Authors |
Shanmugam Manoharan1*, Kaliyaperumal Kolanjiappan1 and Muthukumar Kayalvizhi2 |
Abstract |
This study examined the relationship between oxidative stress and
enzymic antioxidant status in the erythrocytes of thirty-two adult cervical cancer
patients and an equal number of age-matched cervicitis patients and healthy
subjects. Lipid peroxidation was significantly increased, while the activities of
enzymic antioxidants (superoxide dismutase, catalase, glutathione peroxidase)
and glucose-6-phosphate dehydrogenase were decreased in the erythrocytes of
cervical cancer patients as compared to healthy subjects and cervicitis patients.
Thus, this study has demonstrated elevated lipid peroxidation and impaired
enzymic antioxidant activities in the erythrocytes of cervical cancer patients. |
Address and Contact Information |
1Department of Biochemistry, Faculty of Science, Annamalai University,
Annamalainagar - 608 002, India, 2Department of Biochemistry, Ponnaiyah
Ramajayam College, Thanjavur, India
* Corresponding author; tel: + 91-4144-238343 (off.), + 91-4144-249355 (res.),
fax : + 91-4144-238145, e-mail: manshisak@yahoo.com
|
|
Volume 9 (2004) pp 709-721 |
Title |
GREEN TEA MODULATION OF THE BIOCHEMICAL AND ELECTRIC PROPERTIES OF RAT LIVER CELLS THAT
WERE AFFECTED BY ETHANOL AND AGING |
Authors |
Izabela Dobrzyńska1, Anna Śnieciłska2, Elżbieta Skrzydlewska2* and Zbigniew Figaszewski1,3 |
Abstract |
The oxidative stress induced by chronic ethanol consumption,
particularly in concert with the aging process, has been implicated in changes in
the structure and functions of liver cell components including membrane
phospholipids. To counteract such changes, particularly those resulting from
lipid peroxidation, antioxidants may be applied. Green tea contains large
amounts of polyphenols, mainly catechins, which possess antioxidant properties.
The aim of this study was to estimate the efficacy of green tea's influence on the
physicochemical and biochemical properties of the rat liver as affected by the
aging process and/or chronic ethanol intoxication. Several methods were used to
evaluate this effect. Antioxidant properties were evaluated by vitamin E and
antioxidant status determination. The liver trigliceride and cholesterol levels
were also estimated. The extent of lipid peroxidation was determined by
measuring the level of lipid peroxidation products as thiobarbituric reactive
substances (TBARS). The surface charge density of the rat liver cells was
measured using electrophoresis. The concentration of the marker enzymes of
liver damage (alanine aminotransferase and aspartate aminotransferase) in the
blood serum was also evaluated. Relative to the controls, aging was found to
cause a decrease in the liver's antioxidant abilities and provoke an increase in the
level of lipid peroxidation; it also increased the surface charge density of the rat
liver cell membrane. Ethanol significantly aggravated these changes. This might
have resulted in the liver cell membrane damage visible as a leak of alanine
aminotransferase and aspartate aminotransferase into the blood. The ingestion of
green tea with ethanol partially prevented these aging and/or ethanol-induced
changes. Long-term drinking of green tea partially prevents the changes in the
structure and function of the cell membrane caused by chronic ethanol
intoxication. |
Address and Contact Information |
1Institute of Chemistry, University of Białystok, Al. Piłsudskiego 11/4, 15-443
Białystok, 2Department of Analytical Chemistry, Medical Academy of
Białystok, ul. Mickiewicza 2, 15-230 Białystok, 3Laboratory of Interfacial
Electrochemistry, Faculty of Chemistry, University of Warsaw, Poland
* Corresponding autor; tel. (fax): +48-857485707, e-mail: skrzydle@amb.edu.pl |
|
Volume 9 (2004) pp 723-737 |
Title |
TALIN DISTRIBUTION DURING THE DIFFERENTIATION OF SATELLITE CELLS ISOLATED FROM RAT SKELETAL MUSCLE |
Authors |
Edyta Brzóska, Edyta Wróbel, Iwona Grabowska and Jerzy Moraczewski |
Abstract |
Satellite cells (myogenic stem cells) dissociated from adult muscle
tissue proliferate, fuse and form multinucleate myotubes when placed in
culture. This study focused on the role of talin distribution during the
differentiation of satellite cells. Talin plays a key role in anchoring actin
filaments to integrins as well as to the plasma membrane in focal contacts. We
demonstrated that there is a colocalization of talin and phosphoserine residues
during the differentiation of satellite cells, and that it changes after TPA
(a protein kinase C activator) treatment, and showed that talin existing in the
cell-extracellular matrix and cell-cell contact area was not phosphorylated. In
the presence of TPA (24 and 48 h exposure) the level of colocalization of both
talin and phosphoserine residues was the same in the treated cells and in the
control cells, but the level of talin phosphorylation was higher in the treated
cells. We found that in myotubes from TPA treated cultures (144 h exposure to
TPA), talin had localized near the cell membrane in the absence of
phosphoserine residues, and that the level of talin phosphorylation was lower
than in the control cells. We also demonstrated that the expression of talin
during satellite cell differentiation was constant in both the control and TPAtreated
cells. |
Address and Contact Information |
Department of Cytology, Faculty of Biology, Warsaw University,
ul. Miecznikowa 1, 02-096 Warszawa, Poland |
|
Volume 9 (2004) pp 739-753 |
Title |
MOLECULAR CHARACTERISATION OF THE SAND PROTEIN FAMILY:
A STUDY BASED ON COMPARATIVE GENOMICS, STRUCTURAL BIOINFORMATICS AND PHYLOGENY |
Authors |
Amanda Cottage1, Lisa Mullan1, Miriam B.D. Portela1, Elizabeth Hellen1, Tim Carver1, Sunil Patel2, Tanya Vavouri1,
Greg Elgar1 and Yvonne J.K. Edwards1* |
Abstract |
The activities of vertebrate lysosomes are critical to many essential
cellular processes. The yeast vacuole is analogous to the mammalian lysosome
and is used as a tool to gain insights into vesicle mediated vacuolar/lysosome
transport. The protein SAND, which does not contain a SAND domain (PFAM
accession number PF01342), has recently been shown to function at the
tethering/docking stage of vacuole fusion as a critical component of the vacuole
SNARE complex. In this publication we have identified SAND in diverse
eukaryotes, from single celled organisms such as the yeasts to complex multicellular
chordates such as mammals. We have demonstrated subfamily divisions
in the SAND proteins and show that in vertebrates, a duplication event gave rise
to two SAND sequences. This duplication appears to have occurred during early
vertebrate evolution and conceivably with the evolution of lysosomes. Using
bioinformatics we predict a secondary structure, solvent accessibility profile and
protein fold for the SAND proteins and determine conserved sequence motifs,
present in all SAND proteins and those that are specific to subsets. A
comprehensive evaluation of yeast and human functional studies in conjunction
with our in silico analysis has identified potential roles for some of these motifs. |
Address and Contact Information |
11MRC Rosalind Franklin Centre for Genomic Research, Genome Campus,
Hinxton, Cambridge, CB10 1SB, UK, 2Accelrys Inc., 334 Cambridge Science
Park, Milton Road, Cambridge, CB4 0WN, UK
* Corresponding author; e-mail: yjedward@rfcgr.mrc.ac.uk |
|
Volume 9 (2004) pp 755-763 |
Title |
THE AMINOESTERS AS INHIBITORS OF PLASMA MEMBRANE H+-ATPase IN THE YEAST Saccharomyces cerevisiae |
Authors |
Ewa Obłąk1, Tadeusz M. Lachowicz1, Jacek Łuczyński2 and Stanisław Witek2 |
Abstract |
A set of oxalates of a-dimethylamino fatty acids n-alkyl esters
(MEM-ns and n-MEM-8s) and n-dodecyl-N,N-dimethylalaninate (DMAL-12s)
were synthesized. Their activities on the growth, transport, and ATPases from
the yeast Saccharomyces cerevisiae were compared. The compounds differ in
the number of carbon atoms in their aliphatic chain and in the position of that
chain in their molecular structure. The tested aminoesters with twelve carbon
atoms (MEM-10s and DMAL-12s) appeared to have the highest level of activity.
These drugs inhibited plasma membrane H+-ATPase, but no inhibition of
mitochondrial ATPase was observed. Under nitrogen-derepressed conditions, the
aminoesters inhibited amino acid uptake by yeast cells. |
Address and Contact Information |
1Institute of Genetics and Microbiology, University of Wrocław,
Przybyszewskiego 65/73, 51-148 Wrocław, Poland, 2Department of Chemistry,
Wrocław University of Technology, 51-148 Wrocław, Poland |
|
Volume 9 (2004) pp 765-777 |
MEDICAL AND BIOLOGICAL SCIENCES:
TOWARDS A HEALTHIER WORLD Suplement 2: Late Abstracts |
Abstracts List |
DETECTION OF BOVINE LEUKEMIA VIRUS PROVIRAL DNA IN
YAROSLAVSL, MONGOLIAN AND BLACK PIED CATTLE BY PCR
M. Reza Mohammadabadi, Gadji Omarovich Shaikhaev,
Galina Efimovna Sulimova, Obydur Rahman
and M. Reza Mozafari
- p766
DISTRIBUTION OF DIVALENT ZINC AND COPPER IONS IN THE
RAT BRAIN SYNAPTOSOMES: INFLUENCE OF CALCIUM
DEFICIENCY AND DEPOLARISATION
Seyyed Moazam Mortazavi, Mohsen Ani,
Manochehr Mesri Pour and M. Reza Mozafari
- p769
POSTNATAL HYDROCEPHALIC CSF PROMOTES
DIFFERENTIATION OF CORTICAL PROGENITOR CELLS
Mohammad Nabiyouni and Jaleel Miyan
- p772
AN ANALYSIS OF INJURY RISK IN THE MANCHESTER UNITED
TEAM WHEN PLAYING AT HOME AND AWAY
Nader Rahnama, Thomas Reilly and Adrian Lees
- p773
PATTERN OF EXPRESSION OF THE EUKARYOTIC INITIATION
FACTOR 4E (EIF4E) IN SKIN CANCER
Zivar Salehiand Farhad Mashayekhi
- p776
|
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